####
\data includes raw data recording: 
	#(1) sampling conditions, and sample barcoding from field campaigns from July 2024 and Oct 2024 field collection of soil, tripod-mounted bioaerosol sampling, and uncrewed aircraft system (UAS)-mounted bioaerosol sampling for Coccidioides sp., 
	#(2) DNA extraction yields via qiagen powersoil DNA extraction kit with 50ul elution volume, 
	#(3) qPCR detection using the CocciENV assay for coccidioides sp., Bowers et al, 2019
	#(4)  presence/absence  call for Coccidioides using  cocciENV assay, defined as >3 of 4 technical replicates below 40 cycle threshold (<40ct) via quantitative PCR (qPCR)
\collection processing: J. Markweise, K. Bistolas, J. Reichman, J. Head, S. Dobson, M. Radosovich, L. Couper, S. Kruger 4/25

####
BarcodeID	Sample unique identifier/Barcode ID
SiteClusterNumber	Denotes general location of sample (e.g. clusters sampled near others)
SoilMoisture	water content of soil collected as %; NA: represent bioaerosol samples, with no soil moisture data relevant
Type	Type of sample collected, where Precinct: within rodent burrow, Surface: surface near rodent burrow, UAS_filt: uncrewed aircraft system-mounted vacuum pump PTFE filters ground_filt: tripod-mounted vacuum pump PTFE filters
survey_x	sample collection longitude
survey_y	sample collection latitude
elevation_Garmin	sample collection elevation
SampSeason	smpling season (July/October, 2024)
Dataset	differentiates soil samples from aerial samples (UAS, unmanned aircraft)
Blanks	identifies lab or field blanks
DNA.conc.ngul	quantity of extracted DNA in ng/ul, as measured via qubit. TL indicates TOO LOW as measured by qubit high sensitivity reagent.
DNA.conc.ngulzero	quantity of extracted DNA in ng/ul, as measured via qubit. 0 indicates TOO LOW as measured by qubit high sensitivity reagent.
DNA_tot_eluted	total quantity of extracted DNA in ng/ul, as measured via qubit, scaled to 50ul of eluted DNA
standard	R2 standard curve measured by qPCR 
techreps	number of technical replicates run per sample
less40ct	number of technical replicates with <40ct ("detectable")
Call	Positive sample for Coccidioides, per CocciENV assay, Bowers 2019. Call defines positive samples, where 0 denotes negative samples and 1 denotes positive samples for presence of Cocciodoides sp. copia oligo, defined as any sample with >3 of 4 technical replicates with <40ct (cycle threshold) via qPCR (see Bowers et al. 2019 assay for further methods specificity) 
percent_amplified	% of technical replicates amplified
ave_totquant	average relative quantity (standardized oligos)
ave_ct	average relative ct (cycle threshold, i.e. PCR cycle at which oligo with target fluorophore becomes detectable)
Ct1	Ct: qPCR cycle threshold of technical replicate 1
estcn1	estimated number of genetic copies of Coccidioides sp. copia open reading frame, estimated via cocciENV assay qPCR standard curve, technical replicate 1 
Ct2	Ct: qPCR cycle threshold of technical replicate 2
estcn2	estimated number of genetic copies of Coccidioides sp. copia open reading frame, estimated via cocciENV assay qPCR standard curve, technical replicate 2
Ct3	Ct: qPCR cycle threshold of technical replicate 3
estcn3	estimated number of genetic copies of Coccidioides sp. copia open reading frame, estimated via cocciENV assay qPCR standard curve, technical replicate 3
Ct4	Ct: qPCR cycle threshold of technical replicate 4
estcn4	estimated number of genetic copies of Coccidioides sp. copia open reading frame, estimated via cocciENV assay qPCR standard curve, technical replicate 4

####
molecular QC: DNA was quantified via qubit high sensitivity fluorophores and DNA standards and nuclease free water were evaluated as a positive and negative controls per batch of 40 samples, respectively. Field blanks (PTFE filters) and laboratory blanks were extracted and evaluated under congruent conditions as samples. qPCR cocciENV was performed with no-template controls (negatives) and synthetic oligo standards (positives between 3.7*10^2-3.7*10^8)  to quantitatively estimate the copy number of copia open reading frames (e.g. “genetic fragments”) of Coccidioides sp. per Bowers et al, 2019. qPCR with an R2<0.95 were omitted from downstream analyses and qPCR was repeated.